Development of FISH probes for the detection of microorganisms in situ in a double-sided labeling system using an external, universal oligonucleotide

            Project manager: professor Tomasz Gosiewski
            Implementation period: 2023-2024
           

The aim of the study will be to develop a set of fluorescence in situ hybridization (FISH) probes for precise and sensitive detection of bacteria and fungi in samples, which will require only a single, external oligonucleotide, labeled on both sides with a fluorophore. This approach should lead to FISH technology that does not require the high costs of labeling separate probes. The universal, external and tagged nucleotide will be complementary to each new FISH probe.