Application of next-generation sequencing method for the analysis of intestinal mycobiotics in obese rats subjected to transcranial direct current stimulation

Project manager: Dr. Katarzyna Talaga-Ćwiertnia
Implementation period: 2022 - 2023

The main objective of this study is to qualitatively and quantitatively evaluate the intestinal mycobiota of rats in relation to the diet used and the type of stimulation of brain centers. Previous literature data suggest that the qualitative and quantitative composition of the gut microbiota may influence the development of lifestyle diseases, including obesity due to energy dyshomeostasis. Currently, research is being carried out to learn more about bacterial profiles in the course of various diseases, while fungi, which are also an important component of the microbiota, are being overlooked. Hence, the planned scientific outcome will be to determine the fungal profiles in rat intestinal biopsy and feces depending on the degree of fatness and the applied transcranial direct current stimulation (tDCS). The practical goal of the study will be to evaluate the relationship between intestinal mycobiom composition, obesity and transcranial stimulation. The knowledge gained will contribute to a better understanding of the etiopathogenesis of obesity and the potential application of the novel tDCS method for the future treatment of obesity in humans.